青云英语翻译

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Summary objective: based on basic theories of Chinese medicine and TCM clinical basis for construction of recombinant eukaryotic expression vector of HBV x protein pIRES2-AcGFP-HBx. Method: design and synthesis of HBx gene-specific PCR primers, PCR amplification gain HBx gene sequence; the HBx gene

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a摘要 目的:基于中医基础理论与中医临床基础,构建重组HBV X蛋白的真核表达载体pIRES2-AcGFP-HBx。方法:设计合成HBx基因的特异性PCR引物, PCR扩增获得HBx基因序列;将HBx基因序列克隆入T载体(pGEM-T-HBx);再用限制性内切酶BglII和EcoRⅠ双酶切下目的片段,将其亚克隆入真核表达载体pIRES2-AcGFP;双酶切(BglII和EcoRⅠ)和序列测定鉴定重组子;脂质体包裹pIRES2-AcGFP-HBx转染入HepG2细胞,G418筛选得到稳定表达HBx的细胞克隆, Western-blot检测HBx蛋白在转染细胞中的表达情况。结果: PCR扩增获得全长HBx基因序列;双酶切筛选得到阳性重组 [translate]